Journal: Frontiers in Physiology

Article Title: Sex Differences in Metabolic and Behavioral Responses to Exercise but Not Exogenous Osteocalcin Treatment in Mice Fed a High Fat Diet

doi: 10.3389/fphys.2022.831056

Figure Lengend Snippet: Glucose tolerance tests. At 12 weeks of age after 8 weeks of control diet (Control) or high fat diet (HFD); (A) male, (B) female time-courses, (C) area under the curve. Mice at 20 weeks of age following 8 weeks of treatments (D–F) , sedentary mice on control diet with daily saline injection (Control), sedentary mice on HFD (HFD Sed Sal), exercised mice on HFD (HFD Ex Sal) or sedentary mice on HFD treated with osteocalcin (HFD Sed Ocn). (D) Male mice, (E) female time-courses, and (F) area under the curve. Error bars represent standard deviation from the mean; 8-week GTT male groups n = 6, female groups n = 5; end of treatment GTT Male groups n = 5, female groups n = 7. *Indicates significant differences after Bonferroni correction, unadjusted p values are shown above bars.

Article Snippet: Serum mouse osteocalcin concentrations were determined using ELISAs specific to mouse carboxylated osteocalcin (Gla-OC MK127, Takara Bio United States, Mountain View, CA, United States) and uncarboxylated osteocalcin (Glu-OC MK129, Takara Bio).

Techniques: Injection, Standard Deviation

Journal: Frontiers in Physiology

Article Title: Sex Differences in Metabolic and Behavioral Responses to Exercise but Not Exogenous Osteocalcin Treatment in Mice Fed a High Fat Diet

doi: 10.3389/fphys.2022.831056

Figure Lengend Snippet: Metabolic measures after 8 weeks of treatments.

Article Snippet: Serum mouse osteocalcin concentrations were determined using ELISAs specific to mouse carboxylated osteocalcin (Gla-OC MK127, Takara Bio United States, Mountain View, CA, United States) and uncarboxylated osteocalcin (Glu-OC MK129, Takara Bio).

Techniques:

Journal: Frontiers in Physiology

Article Title: Sex Differences in Metabolic and Behavioral Responses to Exercise but Not Exogenous Osteocalcin Treatment in Mice Fed a High Fat Diet

doi: 10.3389/fphys.2022.831056

Figure Lengend Snippet: Progressive problem-solving in puzzle box. Latencies for males and females of each treatment group during the puzzle box test (A–D) squares represent data from males, triangles represent data from females. (A) Controls; (B) High fat diet, sedentary with daily saline injections (HFD Sed Sal); (C) High fat diet, daily exercise and saline injections (HFD Ex Sal); (D) High fat diet sedentary with daily osteocalcin injections (HFD Sed Ocn); (E) Problem-solving scores, Z-normalized to the male control group. Data point and bars represent mean ± standard deviation; male control n = 10, HFD groups n = 9; female control n = 10, HFD n = 12. Statistically significant differences after Bonferroni correction indicated by * with unadjusted p values shown above bars.

Article Snippet: Serum mouse osteocalcin concentrations were determined using ELISAs specific to mouse carboxylated osteocalcin (Gla-OC MK127, Takara Bio United States, Mountain View, CA, United States) and uncarboxylated osteocalcin (Glu-OC MK129, Takara Bio).

Techniques: Standard Deviation

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Antiosteoporotic Effect of Combined Extract of Morus alba and Polygonum odoratum

doi: 10.1155/2014/579305

Figure Lengend Snippet: Effect of various doses of the combined extract of Morus alba and Polygonum odoratum leaves on the levels of serum osteocalcin ( n = 6/group). *** P value < 0.001, compared to OVX: vehicle treated group; # P value <.05, OVX compared to control group. D1: the combined extract at dose of 5 mg · kg −1 BW, D2: the combined extract at dose of 150 mg · kg −1 BW, and D3: the combined extract at dose of 300 mg · kg −1 BW.

Article Snippet: The osteocalcin level was determined by using Rat Gla-Osteocalcin High Sensitive EIA Kit (Takara Bio Inc. Company, Japan).

Techniques: